EB05051 Goat Anti-AKAP12 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AKAP12; A kinase (PRKA) anchor protein (gravin) 12; GRAVIN; AKAP250; kinase scaffold protein gravin; A kinase (PRKA) anchor protein 12; DKFZp686M0430; DKFZp686O0331
Official Symbol: AKAP12
Accession Number(s): NP_005091.2; NP_653080.1
Human GeneID(s): 9590
Gene Ontology Terms: protein kinase A anchoring activity; protein transporter activity; protein targeting; G-protein coupled receptor protein signaling pathway; cytoplasm
Important Comments: This anrtibody is expected to recognize both reported isoforms. Immunogen Peptide with sequence C-KQERESAKSELTES, from the C Terminus of the protein sequence according to NP_005091.2; NP_653080.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 250kDa band observed in lysates of cell line HeLa (calculated MW of 191kDa according to NP_005091.2). Recommended concentration: 0.03-0.1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Lynch et al, RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem. 2005 Sep 30;280(39):33178-89. Epub 2005 Jul 19.
PMID: 16030021
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05051 (0.03µg/ml) staining of HeLa cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
