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Functional Clustering

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  • Everest Biotech Ltd Goat Anti-ALDH1A1 (C Terminus) Antibody
    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-ALDH1A1 (C Terminus) Antibody

    EB05049-T Goat Anti-ALDH1A1 (C Terminus) Antibody - Trial Size
    Price: $100.00 / €100,00 / £75.00
    Size: 20µg specific antibody in 40µl
    EU Availability: In stock
    US Availability: In stock
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    EB05049 Goat Anti-ALDH1A1 (C Terminus) Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
    Click to add to EURO Shopping Cart (EU customers only)
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    Cart for UK (GBP) customers coming soon, meanwhile click for ordering info

    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: ALDH1A1; aldehyde dehydrogenase 1 family, member A1; ALDC; ALDH-E1; ALDH1; ALDH11; MGC2318,; PUMB1; RALDH1; ALDH class 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase 1A1; aldehyde dehydrogenase, liver cytosolic; retinal dehydrogenase 1
    Official Symbol: ALDH1A1
    Accession Number(s): NP_000680.2
    Human GeneID(s): 216
    Gene Ontology Terms: aldehyde dehydrogenase (NAD+) activity; androgen binding; electron transporter activity; aldehyde metabolism; cytosol; oxidoreductase activity
    Important Comments: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

    Immunogen

    Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 55kDa band observed in human liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.03-0.1µg/ml.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human, Mouse, Rat, Dog

    Product Specific Reference

    The goat polyclonal antibody used in these papers was manufactured by us:

    Iwata M. Retinoic acid imprints gut-homing specificity on T cells. Immunity. 2004 Oct;21(4):527-38.
    PMID: 15485630

    Yoon M et al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of
    liver and lung development. Toxicol Sci. 2006 Feb;89(2):386-98. Epub 2005 Nov 16.
    PMID: 16291827
     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    Trial Size Available


    EB05049 Goat Anti-ALDH1A1 (C Terminus) Antibody

    EB05049 staining (0.03µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


    EB05049 Goat Anti-ALDH1A1 (C Terminus) Antibody

    HEK293 overexpressing ALDH1A1 (RC200723) and probed with EB05049 (mock transfection in first lane), tested by Origene.

    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-ALDH1A1 (C Terminus) Antibody