EB05048 Goat Anti-Tissue Factor Pathway Inhibitor Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: TFPI; EPI; LACI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) Official Symbol: TFPI Accession Number(s): NP_006278.1 Human GeneID(s): 7035 Gene Ontology Terms: serine protease inhibitor activity; blood coagulation Important Comments: This antibody is expected to recognize isoform a (NP_006278.1) only. Immunogen Peptide with sequence C-VKIAYEEIFVKNM, from the C Terminus of the protein sequence according to NP_006278.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kDa according to NP_006278.1). Recommended concentration: 0.3-1µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human Background ReferenceWun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze GJ Jr. Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988 May 5;263(13):6001-4 PMID: 2452157
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB05048 staining (0.3µg/ml) of Hela lysate (RIPA buffer, 35µg total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |