EB05035-T Goat Anti-Aurora kinase A / AURKA Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB05035 Goat Anti-Aurora kinase A / AURKA Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AURKA; aurora kinase A; AIK; ARK1; AURA; AURORA2; BTAK; MGC34538; STK15; STK6; STK7; IPL1-related kinase; aurora-A; aurora-related kinase 1; aurora/IPL1-like kinase; breast-tumor-amplified kinase; serine/threonine kinase 15; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6
Official Symbol: AURKA
Accession Number(s): NP_003591.2
Human GeneID(s): 6790
Gene Ontology Terms: cAMP-dependent protein kinase activity; protein kinase CK2 activity; protein tyrosine kinase activity; ATP binding; protein serine/threonine kinase activity; cell cycle; mitosis; protein amino acid phosphorylation; oncogenesis; spindle; spindle pole body; nucleus; transferase activity
Immunogen Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceZhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S.
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
Nat Genet 1998 Oct;20(2):189-93
PMID: 9771714
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05035 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB05035 in the right panel (vector-only transfection in first and fourth lanes). |
