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Functional Clustering

  • Apoptosis and its regulation
  • Lipid Transport
  • DNA Metabolism and Repair
  • GTPase mediated Signal Transduction
  • Metal ion binding / Zinc finger
  • Transcription & its regulation
  • Neuronal development / differentiation
  • RNA binding / splicing / processing
  • Protein transport / localization & binding
  • Ubiquitin cycle / Protein Metabolism
  • GTP-binding & GTP activation
  • Protein Kinase Activity
  • ATPase activity & ATP binding
  • Wnt signaling pathway
  • Protein Phosphatase Activity
  • Transmembrane Proteins & Receptors
  • Proteolysis / Protein Metabolism

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  • Secondary Antibodies

  • Rabbit anti-Goat HRP Conjugated


  • Peptides

  • Immunising Peptides available for all antibodies
  • Everest Biotech Ltd Goat Anti-PPP1R15A / GADD34 Antibody
    Online Catalogue > P114-RHO-GEF to ZNF9 > Goat Anti-PPP1R15A / GADD34 Antibody

    EB05034-T Goat Anti-PPP1R15A / GADD34 Antibody - Trial Size
    Price: $100.00 / €100,00 / £75.00
    Size: 20µg specific antibody in 40µl
    EU Availability: In stock
    US Availability: In stock
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    EB05034 Goat Anti-PPP1R15A / GADD34 Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
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    Cart for UK (GBP) customers coming soon, meanwhile click for ordering info

    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: PPP1R15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A; GADD34; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
    Official Symbol: PPP1R15A
    Accession Number(s): NP_055145.2
    Human GeneID(s): 23645
    Gene Ontology Terms: cell cycle arrest; response to DNA damage stimulus; apoptosis

    Immunogen

    Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.2.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3µg/ml.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human

    Product Specific Reference

    The goat polyclonal antibody used in this paper was manufactured by us:

    Aghi M et al. Effect of chemotherapy-induced DNA repair on oncolytic herpes simplex viral
    replication. J Natl Cancer Inst. 2006 Jan 4;98(1):38-50.
    PMID: 16391370

     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    Trial Size Available


    EB05034 Goat Anti-PPP1R15A / GADD34 Antibody

    EB05034 (0.1µg/ml) staining of HEPG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

    Online Catalogue > P114-RHO-GEF to ZNF9 > Goat Anti-PPP1R15A / GADD34 Antibody