EB05034-T Goat Anti-PPP1R15A / GADD34 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB05034 Goat Anti-PPP1R15A / GADD34 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PPP1R15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A; GADD34; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A Official Symbol: PPP1R15A Accession Number(s): NP_055145.2 Human GeneID(s): 23645 Gene Ontology Terms: cell cycle arrest; response to DNA damage stimulus; apoptosis Immunogen Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us: Aghi M et al. Effect of chemotherapy-induced DNA repair on oncolytic herpes simplex viral replication. J Natl Cancer Inst. 2006 Jan 4;98(1):38-50. PMID: 16391370
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 


EB05034 (0.1µg/ml) staining of HEPG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |